Composite

Part:BBa_I712083

Designed by: Marko Bitenc   Group: iGEM07_Ljubljana   (2007-10-22)

CMV promoter - CD4 (shortened) - TEV C

Human CD4 shortened (without cytosolic domain) receptor fused with C-terminal half of TEV protease and Myc tag. After heterodimerisation with N-terminal half fused with CCR5 coreceptor which occurs at binding of virus, the TEV protease becomes active and is able to recognize and cleave its substrate.
This construct is under control of CMV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798
    Illegal SapI.rc site found at 1915
    Illegal SapI.rc site found at 2225


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Categories
Parameters
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